quantitative reverse transcription (qrt-) pcr Search Results


reverse transcription quantitative polymerase chain reaction (pcr)  (Kaltenbach GmbH)
90
Kaltenbach GmbH reverse transcription quantitative polymerase chain reaction (pcr)
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Reverse Transcription Quantitative Polymerase Chain Reaction (Pcr), supplied by Kaltenbach GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/reverse+transcription+quantitative+polymerase+chain+reaction++pcr+/pmc07327646-16-6-13
Average 90 stars, based on 1 article reviews
reverse transcription quantitative polymerase chain reaction (pcr) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
quantitative reverse transcription pcr-based assay bln assay  (CellSearch inc)
90
CellSearch inc quantitative reverse transcription pcr-based assay bln assay
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Quantitative Reverse Transcription Pcr Based Assay Bln Assay, supplied by CellSearch inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/quantitative+reverse+transcription+pcr+based+assay+bln+assay/pm22090341-38-43-51
Average 90 stars, based on 1 article reviews
quantitative reverse transcription pcr-based assay bln assay - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
reverse transcription quantitative real-time pcr  (ELISA Technologies Inc)
90
ELISA Technologies Inc reverse transcription quantitative real-time pcr
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Reverse Transcription Quantitative Real Time Pcr, supplied by ELISA Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/reverse+transcription+quantitative+real+time+pcr/pmc04220711-101-10-17
Average 90 stars, based on 1 article reviews
reverse transcription quantitative real-time pcr - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
primers for quantitative reverse transcription polymerase chain reaction  (First BASE Laboratories)
90
First BASE Laboratories primers for quantitative reverse transcription polymerase chain reaction
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Primers For Quantitative Reverse Transcription Polymerase Chain Reaction, supplied by First BASE Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/primers+used+for+quantitative+reverse+transcription+polymerase+chain+reaction++qrt+pcr+/10__22159_slash_ijap__2018__v10s1__74-40-0-13
Average 90 stars, based on 1 article reviews
primers for quantitative reverse transcription polymerase chain reaction - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
reverse transcription-quantitative pcr  (Meso Scale Diagnostics LLC)
90
Meso Scale Diagnostics LLC reverse transcription-quantitative pcr
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Reverse Transcription Quantitative Pcr, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/quantitative+reverse+transcription+pcr/pmc11574399-76-6-27
Average 90 stars, based on 1 article reviews
reverse transcription-quantitative pcr - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
validated multiplex quantitative real-time reverse transcription polymerase chain reaction (mqrt-pcr) assays  (HY Labs)
90
HY Labs validated multiplex quantitative real-time reverse transcription polymerase chain reaction (mqrt-pcr) assays
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Validated Multiplex Quantitative Real Time Reverse Transcription Polymerase Chain Reaction (Mqrt Pcr) Assays, supplied by HY Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/validated+multiplex+quantitative+real+time+reverse+transcription+polymerase+chain+reaction++mqrt+pcr++assays/pmc06220521-170-31-51
Average 90 stars, based on 1 article reviews
validated multiplex quantitative real-time reverse transcription polymerase chain reaction (mqrt-pcr) assays - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
mrna quantitative reverse transcription pcr (rt-qpcr)  (AllBio Science Inc)
90
AllBio Science Inc mrna quantitative reverse transcription pcr (rt-qpcr)
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Mrna Quantitative Reverse Transcription Pcr (Rt Qpcr), supplied by AllBio Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/mrna+quantitative+reverse+transcription+pcr++rt+qpcr+/pm27698264-73-7-17
Average 90 stars, based on 1 article reviews
mrna quantitative reverse transcription pcr (rt-qpcr) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
denv-chikv multiplex real-time quantitative reverse transcription polymerase chain reaction (rt-pcr) assay  (AITBIOTECH Pte Ltd)
90
AITBIOTECH Pte Ltd denv-chikv multiplex real-time quantitative reverse transcription polymerase chain reaction (rt-pcr) assay
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Denv Chikv Multiplex Real Time Quantitative Reverse Transcription Polymerase Chain Reaction (Rt Pcr) Assay, supplied by AITBIOTECH Pte Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/denv+chikv+multiplex+real+time+quantitative+reverse+transcription+polymerase+chain+reaction++rt+pcr++assay/pmc06231660-64-6-23
Average 90 stars, based on 1 article reviews
denv-chikv multiplex real-time quantitative reverse transcription polymerase chain reaction (rt-pcr) assay - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
real-time quantitative reverse transcription pcr facility  (MJ Research)
90
MJ Research real-time quantitative reverse transcription pcr facility
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Real Time Quantitative Reverse Transcription Pcr Facility, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/real+time+quantitative+reverse+transcription+pcr+facility/10__1097_slash_mib__0000000000001129-88-10-16
Average 90 stars, based on 1 article reviews
real-time quantitative reverse transcription pcr facility - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rna extraction kit, reverse transcription quantitative real-time pcr kit  (ELK Biotechnology)
90
ELK Biotechnology rna extraction kit, reverse transcription quantitative real-time pcr kit
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Rna Extraction Kit, Reverse Transcription Quantitative Real Time Pcr Kit, supplied by ELK Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/rna+extraction+kit++reverse+transcription+quantitative+real+time+pcr+kit/pm37459672-32-72-82
Average 90 stars, based on 1 article reviews
rna extraction kit, reverse transcription quantitative real-time pcr kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
quantitative reverse transcription–pcr qrt–pcr kappafirst peqlab#07kk600-03  (PEQLAB)
90
PEQLAB quantitative reverse transcription–pcr qrt–pcr kappafirst peqlab#07kk600-03
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Quantitative Reverse Transcription–Pcr Qrt–Pcr Kappafirst Peqlab#07kk600 03, supplied by PEQLAB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/quantitative+reverse+transcription+pcr+qrt+pcr+kappafirst+peqlab+07kk600+03/pmc04366489-259-5-9
Average 90 stars, based on 1 article reviews
quantitative reverse transcription–pcr qrt–pcr kappafirst peqlab#07kk600-03 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
primers for stem-loop reverse transcription pcr (rt-pcr) and quantitative pcr (qpcr)  (Ribobio co)
90
Ribobio co primers for stem-loop reverse transcription pcr (rt-pcr) and quantitative pcr (qpcr)
iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 <t>PCR</t> array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative <t>polymerase</t> chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.
Primers For Stem Loop Reverse Transcription Pcr (Rt Pcr) And Quantitative Pcr (Qpcr), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+(qrt-)+pcr/primers+for+stem+loop+reverse+transcription+pcr++rt+pcr++and+quantitative+pcr++qpcr+/pmc04363339-43-7-24
Average 90 stars, based on 1 article reviews
primers for stem-loop reverse transcription pcr (rt-pcr) and quantitative pcr (qpcr) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 PCR array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative polymerase chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.

Journal: Haematologica

Article Title: Transforming activities of the NUP98-KMT2A fusion gene associated with myelodysplasia and acute myeloid leukemia

doi: 10.3324/haematol.2019.219188

Figure Lengend Snippet: iNUP98-KMT2A expression impairs cell cycle progression of murine embryonic fibroblasts and bone marrow-derived hematopoietic stem and progenitor cells. (A) Murine embryonic fibroblasts (MEF), derived from iNUP98-KMT2A and wildtype (WT) control (CTRL) littermate mice were cultured in vitro in the presence of doxycycline (DOX) (1 μg/mL). iNUP98-KMT2A expression is shown relative to the level of GAPDH expression. * P <0.05, unpaired t -test, n=3. (B) Flow cytometry-based cell cycle analysis showed increased G 1 and decreased G 2 /M fractions of in vitro -cultured iNUP98-KMT2A+ MEF compared to WT controls. * P <0.05, unpaired t -test, n=3. (C) iNUP98-KMT2A and WT MEF cultured for eight passages in the presence of DOX (1 μg/mL) were stained for senescence-associated B-galactosidase activity with X-Gal (left panel). The number of X-Gal + cells in the culture was quantified (right panel). Images and counts are representative of three biological replicates. Scale bars: 100 μm. ** P <0.01, unpaired t-test, n=3. (D) Differential mRNA expression from early (passages 1-2) and late (passages 8-10) passaged WT and iNUP98-KMT2A MEF analyzed by a RT2 PCR array. Significant ( P <0.05) changes are highlighted in red. (E) Validation of differentially expressed genes in MEF by quantitative polymerase chain reaction analysis in WT and iNUP98-KMT2A hematopoietic stem and progenitor cells after exposure to DOX (1 μg/mL) in vitro for 48 h. *<0.05, unpaired t -test, n=3.

Article Snippet: Using fluorescent in situ hybridization and reverse transcription quantitative polymerase chain reaction (PCR), Kaltenbach et al . found that inv(11)(p15q23) leads to fusion of the NUP98-FG -repeats to almost the entire KMT2A open reading frame (ORF).

Techniques: Expressing, Derivative Assay, Control, Cell Culture, In Vitro, Flow Cytometry, Cell Cycle Assay, Staining, Activity Assay, Biomarker Discovery, Real-time Polymerase Chain Reaction